Implications of the prevalence of Ascaris sp. in the funerary context of a Late Antique population (5th-7th c.) in Granada (Spain).

OBJECTIVE: To evaluate the prevalence of gastro-intestinal parasites in human remains from Late Antiquity (5th - 7th c.) Granada (Spain). MATERIALS: The study included pelvic and cranial control samples from 17 skeletons from the archaeological sites of Los Mondragones (n = 13) and Rafael Guillén (n = 4). METHODS: In the paleoparasitological study, soil samples from pelvic area and cranium were analyzed using the rehydration, homogenization, and micro-sieving method and visualization under brightfield microscopy. RESULTS: Ascaris sp. eggs were detected in pelvic samples from seven individuals. CONCLUSIONS: These findings may indicate that this parasite was endemic. Its detection frequency is one of the highest reported at group level in an osteological series from Late Antiquity. SIGNIFICANCE: The prevalence of Ascaris sp. associated with skeletal remains has implications for assessing the lifestyle and health of populations in southern Spain during the Late Antique period. LIMITATIONS: The number of individuals is small and taphonomic processes could have limited paleoparasitological findings SUGGESTIONS FOR FURTHER RESEARCH: Future interdisciplinary studies of this type are warranted in larger osteological series to improve knowledge of parasitosis in the past.

Zoonotic parasite infection from a funerary context: A Late Antique child case from Cantabrian Spain.

OBJECTIVE: To evaluate the presence of Dicrocoelium sp. in a child from a Late Antique funerary context from Cantabrian Spain and discuss whether the infection is true infection or pseudoparasitosis. MATERIALS: Four skeletons, including one from a 5-7 year old child, have been analysed from the archaeological site of El Conventón, dated between the sixth and seventh centuries AD. METHODS: The paleoparasitological study was conducted through the analysis of soil samples from different parts of the skeleton and funerary context using the rehydration, homogenization, and micro-sieving method, and visualized through brightfield microscopy. RESULTS: A soil sample from the pelvic region tested positive for Dicrocoelium sp. (possibly D. dendriticum). CONCLUSIONS: The child was infected with Dicrocoelium dendriticum, which based on archaeological and historical contexts may be related to hygiene or dietary behaviour. SIGNIFICANCE: We present one of the few cases of the identification of a Dicrocoelidae parasite directly associated with a human skeleton that provides historical knowledge of a zoonotic disease. LIMITATIONS: The diagnosis of a zoonosis through the identification of ancient parasites is complex. In addition, Dicrocoelium sp. in association with skeletal human remains is rare due to the potential low prevalence of this parasite. SUGGESTIONS FOR FURTHER RESEARCH: Highlight the importance of paleoparasitological analysis to link parasitic infection diseases with socioeconomic issues by using funerary contexts with skeletal remains.

Two cases of pelvic trauma with survival in the necropolis of Qubbet el-Hawa (Egypt).

OBJECTIVE: To evaluate the presence of a trauma that results in pelvic ring disruption and its clinical implications in two individuals from ancient Egypt. MATERIALS: Two complete skeletons of adult women, dated to the Middle Kingdom (c. 1980-1760 BCE), from two tombs in the necropolis of Qubbet el-Hawa (Aswan, Egypt). METHODS: The anatomical elements were examined macroscopically in Egypt. RESULTS: Unilateral sacroiliac luxation with disruption of the pubic symphysis was detected in both individuals. The presence of an overlapped symphysis was evident in female QH34aa. A healed fracture of the iliopubic ramus are present in female QH122. Antemortem bone alterations in the pelvis demonstrate that they survived the trauma. CONCLUSIONS: Pelvic trauma due to a lateral compression was detected in the os coxae. Both women survived but probably had severe sequelae that impaired their quality of life. At least one of them might have received some type of medical treatment. SIGNIFICANCE: Both cases shed light on pelvic fractures, their mechanisms of production, and their effects on bone and its functionality, as well as revealing possible associated lesions of soft tissues and vital organs. LIMITATIONS: The pelvis, or at least the pubic symphysis, must be well preserved to allow the correct diagnosis of this type of lesion. SUGGESTIONS FOR FURTHER RESEARCH: Studies are warranted on the detection of this type of fracture, focusing on the subtle bone changes that indicate its presence. There is also a need to develop methodologies that combine the study of bone and soft tissue alterations.

At the beginnings of the funerary Megalithism in Iberia at Campo de Hockey necropolis.

The excavations undertaken at the Campo de Hockey site in 2008 led to the identification of a major Neolithic necropolis in the former Island of San Fernando (Bay of Cádiz). This work presents the results of the latest studies, which indicate that the site stands as one of the oldest megalithic necropolises in the Iberian Peninsula. The main aim of this work is to present with precision the chronology of this necropolis through a Bayesian statistical model that confirms that the necropolis was in use from c. 4300 to 3800 cal BC. The presence of prestige grave goods in the earliest and most monumental graves suggest that the Megalithism phenomenon emerged in relation to maritime routes linked to the distribution of exotic products. We also aim to examine funerary practices in these early megalithic communities, and especially their way of life and the social reproduction system. As such, in addition to the chronological information and the Bayesian statistics, we provide the results of a comprehensive interdisciplinary study, including anthropological, archaeometric and genetic data.

The outline of the pubic symphyseal surface is sexually dimorphic and changes with age in humans.

The bony symphyseal surface is an important trait for age-at-death estimation from human skeletal remains. In this paper, we demonstrate for the first time that the outline of the human symphyseal surface is sexually dimorphic and that it changes with age. We present a geometric morphometric analysis based on a sample of 323 symphyseal pubic bones from males and females in the age range of 14 to 82 years. These bones were surface-scanned and the resulting surface models were measured along the ventral and dorsal borders of the symphyseal surface using two fixed and 36 curve semilandmarks. Our findings imply that age-related changes in the outline of the symphyseal surface differ between the sexes. According to our results, age explains 5% of total shape variation in females, but less than 1% in males. These findings for the outline, could potentially complement existing sex and age-at-death estimation methods based on other features of the bony pubic surface.

Correlation of the human pubic symphysis surface with age-at-death: a novel quantitative method based on a bandpass filter.

Age-at-death estimation from skeletal remains typically utilizes the roughness of pubic symphysis articular surfaces. This study presents a new quantitative method adapting a tool from geometric morphometrics, bandpass filtering of partial warp bending energy to extract only age-related changes of the surfaces. The study sample consisted of 440 surface-scanned symphyseal pubic bones from men between 14 and 82 years of age, which were landmarked with 102 fixed and surface semilandmarks. From the original sample, 371 specimens within Procrustes distance of 0.05 of the side-specific average were selected. For this subsample, age was correlated with total bending energy (calculated as summed squared partial warps amplitudes) for a wide range of plausible bandpass filters. For our subsample's 188 right-side surfaces, the correlation between age and bandpass filtered versions of bending energy peaks relatively sharply at r = -0.648 for ages up through 49 years against the first seven partial warp amplitudes only. The finding for left symphyses is similar. The results demonstrate that below the age 50, the symphyseal surface form changes most systematically related to age may be best detected by a lowpass-filtered version of bending energy: signals at the largest geometric scales of roughness rather than its full spectrum. Combining this method with information from other skeletal features could further improve age-at-death estimation based on the symphyseal pubic surface.

A meta-analytic review for the patella sexual dimorphism assessment / Una revisión meta-analítica para la evaluación del dimorfismo sexual de la patela

Sexual dimorphism is one of the most important ways to identify bone remains in mass disasters. Many of them have been used for this purpose; mainly skull, pelvis and long bones. However, only a few studies using the patella have been done and, to our knowledge, there are no assessments of previous results in the literature. Meta-analysis provides a useful strong tool to test, in a systematic way, the most relevant information about a given research field. The aim of this study is to apply the meta-analytic technique to assess the major studies concerning sexual dimorphism in the patella by measuring classical metric traits: maximum height and maximum width, with different techniques, such as caliper, radiography, tomography and magnetic resonance. The 17 papers found, involving a total sample size higher than 2600 patellae, showed a very high heterogeneity- around 93 % of I2 value, for height and width measurements when all the studies were analyzed together. Homogeneity increased when each study was classified according to the techniques used. In this case, a statistical difference appeared, among the several subgroups of techniques for the two measurements, suggesting the importance of the methodology used. Maximum height and maximum width were all showed to be statistically relevant in distinguishing both sexes.

El dimorfismo sexual es una de las formas más importantes para identificar restos óseos en desastres masivos. Se han utilizado huesos como cráneo, pelvis y huesos largos para la diferenciación sexual. Sin embargo, solo se han realizado unos pocos estudios con la patela y, hasta donde sabemos, no hay evaluaciones de resultados anteriores en la literatura. El meta-análisis proporciona una herramienta sólida y útil para probar, de manera sistemática, la información más relevante sobre un cierto campo de investigación. El objetivo de este estudio consiste en aplicar la técnica metaanalítica para evaluar los principales estudios sobre dimorfismo sexual en la patela midiendo los rasgos métricos clásicos: altura máxima y anchura máxima, con diferentes técnicas: calibre, radiografía, tomografía y resonancia magnética. Los 17 de documentos encontrados, con un tamaño de muestra total superior a 2600 patelas, mostraron una heterogeneidad muy alta, alrededor del 93 % del valor de I2, para mediciones de altura y anchura cuando todos los estudios se analizaron juntos. La homogeneidad aumentó cuando cada estudio se clasificó de acuerdo con las técnicas utilizadas. En este caso, se observó diferencias estadísticas, entre los subgrupos de técnicas para las dos mediciones, lo que sugiere la importancia de la metodología utilizada. La altura máxima y la anchura máxima mostraron ser estadísticamente relevantes para distinguir ambos sexos.

Possible interpersonal violence in the Neolithic necropolis of Campo de Hockey (San Fernando, Cádiz, Spain).

OBJECTIVE: To interpret traumatic cranial lesions on two individuals buried at the Neolithic necropolis of Campo de Hockey, Spain. MATERIALS: Skeletal remains and grave goods associated with two individuals recovered in good condition from a single grave. METHODS: Crania were macroscopically and radiographically analysed. RESULTS: Of all the individuals recovered from this site, only two present perimortem skull injuries. Fractures were located on the right side of the skulls, one on the frontal bone and the other on the parietal bone. CONCLUSIONS: The individuals present signs of interpersonal violence. In both cases, the injuries are perimortem and may have contributed to the deaths of the individuals. SIGNIFICANCE: The identification of interpersonal violence in the two individuals buried in the tomb, alongside the archaeological context of the burials, provides unique insight into Neolithic burial practices in the Iberian Peninsula and the role that paleopathology can play in the recognition of social complexity. LIMITATIONS: The skeletal remains of the two individuals were fragmented, rendering the identification of perimortem lesions difficult. SUGGESTIONS FOR FURTHER RESEARCH: New imaging techniques, including CT-scan, will provide more detailed analyses.

Sex determination in a Spanish population based on sacrum.

Sex determination is one of the essential steps when it comes to establishing an individual's biological profile. It is important in both archaeology and forensic studies. The sacrum is not generally conserved, but in cases where it is, it can be used for determination of the sex of skeletal remains. Furthermore, the sacrum is not a commonly studied bone and has not been studied in a Spanish population. For this study, measurements of 170 sacra of individuals from the contemporary osteological collection of San José from Granada including only the adults were taken. Measurements based on the anatomical regions of the sacra were established in order to obtain some regression formulas to determine sex. Our results show that the Superior Transverse Line and Right Lateral Sacral Crest are the most dimorphic structures, achieving a 74% rate of correct classification of sex in a univariate analysis. In multivariate analysis, when the mentioned variables were combined, an 81.41% rate of correct classification was achieved. Our results show that our method can be applied with other methods at the same time to determine the sex of individuals in forensic and archaeological contexts.

Specific-age group sex estimation of infants through geometric morphometrics analysis of pubis and ischium.

Sex determination of unknown individuals is one of the primary goals of Physical and Forensic Anthropology. The adult skeleton can be sexed using both morphological and metric traits on a large number of bones. The human pelvis is often used as an important element of adult sex determination. However, studies carried out about the pelvic bone in subadult individuals present several limitations due the absence of sexually dimorphic characteristics. In this study, we analyse the sexual dimorphism of the immature pubis and ischium bones, attending to their shape (Procrustes residuals) and size (centroid size), using an identified sample of subadult individuals composed of 58 individuals for the pubis and 83 for the ischium, aged between birth and 1year of life, from the Granada osteological collection of identified infants (Granada, Spain). Geometric morphometric methods and discriminant analysis were applied to this study. The results of intra- and inter-observer error showed good and excellent agreement in the location of coordinates of landmarks and semilandmarks, respectively. Principal component analysis performed on shape and size variables showed superposition of the two sexes, suggesting a low degree of sexual dimorphism. Canonical variable analysis did not show significant changes between the male and female shapes. As a consequence, discriminant analysis with leave-one-out cross validation provided low classification accuracy. The results suggested a low degree of sexual dimorphism supported by significant sexual dimorphism in the subadult sample and poor cross-validated classification accuracy. The inclusion of centroid size as a discriminant variable does not imply a significant improvement in the results of the analysis. The similarities found between the sexes prevent consideration of pubic and ischial morphology as a sex estimator in early stages of development. The authors suggest extending this study by analysing the different trajectories of shape and size in later ontogeny between males and females.

Evaluation of the maximum length of deciduous teeth for estimation of the age of infants and young children: proposal of new regression formulas.

The methodology used to identify individuals in forensic anthropology requires a minimum degree of precision and accuracy and should be based on identified and representative samples. Achievement of these objectives in infant skeletons is hampered by the scarcity of appropriate samples. The dental age estimation methods of Liversidge et al. (Am J Phys Anthropol 90: 307-313, 1993) and Deutsch et al. (Growth 49: 207-17, 1985) were applied to the Granada osteological collection of identified infants (Granada, Spain) in order to evaluate its applicability in a Mediterranean population. Significant differences were found between the estimated and real ages in both cases. Based on the measurements obtained in 140 fetuses and infants, new regression formulas were developed to estimate age from the metric study on deciduous teeth. Independent functions are provided for each deciduous maxillary and mandibular tooth in each sex, along with the margin of error (95% confidence interval). These formulas appear to offer one of the best methods available for estimating the age of Mediterranean infants in forensic anthropology settings.

Image analysis of pubic bone for sex determination in a computed tomography sample.

Radiology has demonstrated great utility for sex determination, but most studies are based in metrical and morphological methods in order to perform an identification profile. It is presented an easy image analysis-based method aimed to demonstrate the relationship between the bony tissue ultrastructure and sex by using several grey level histogram variables obtained from computed tomography images using sagittal sections of the pubic symphysis surface and the body of the pubis. The CT sample consisted of 169 hospital DICOM archives of known sex and age. The multivariate binary logistic regression models calculated showed reliabilities for sex determination between 87.4 and 95.8 %, with a high intra and inter-observer agreement. The suggested method is not only considered useful to perform an identification profile during Virtopsy, but even for application in further studies in order to attach a quantitative correlation for tissue ultrastructure characteristics without complex and expensive methods beyond image analysis.

Alcohol intake and risk of aggressive histological basal cell carcinoma: a case-control study.

BACKGROUND: Aggressive basal cell carcinomas (BCC) are not rare. These subtypes of skin cancer are characterized by an infiltrative behavior and rapid progression. Often, management may be difficult. Recent evidence suggests that minimal UV exposure in combination with other behavioral and/or environmental factors may lead to higher incidence of BCC and, therefore, more risk of aggressive subtypes of this malignancy. Alcohol is a very commonly consumed beverage in Western societies, especially in association with outdoors activities. OBJECTIVE: To investigate a possible relationship between alcohol intake and aggressive histological variants of BCC. MATERIALS AND METHOD: We designed a prospective study. Patients who underwent surgery for BCC in our hospital were interviewed to collect data regarding alcohol intake. The specimens were reviewed by a pathologist and classified into aggressive and non-aggressive subtypes. Statistical analysis was performed using SPSS software. RESULTS: 136 patients were included. Of participants with aggressive BCCs, 10 (26.3%) were abstainers, 4 (10.4%) had light consumption, 18 (47.5%) moderate consumption and 6 (15.8%) heavy consumption, while among participants with non-aggressive BCCs, 57 (58.2%) were abstainers, 29 (29.5%) had light consumption, 10 (10.2%) moderate consumption and 2 (2.1%) heavy consumption. In the multivariate analysis we found a positive significant association between alcohol consumption and the presence of aggressive BBCs. CONCLUSIONS: According to our results, alcohol intake may be linked with a higher incidence of aggressive subtypes of BCC.

Age estimation by a dental method: a comparison of Lamendin's and Prince & Ubelaker's technique.

Lamendin et al. (J Forensic Sci 1992;37:1373) developed a general technique to estimate age of adults at death using two dental features: periodontosis and translucency of the tooth root. Prince and Ubelaker (J Forensic Sci 2002;47:107) modified this method, creating a formula for each sex and for different ancestries, and obtained more precise age estimations. In the present study, the validity of each method was tested in 45 males and 34 females of Spanish Caucasian origin, and a novel formula, based on Prince and Ubelaker method, was specifically developed for a population of mixed racial origin (mestizo) from Colombia, and findings obtained were again compared with those yielded by Lamendin adult dental aging technique. The Prince and Ubelaker method proved more accurate than the Lamendin technique in the Spanish Caucasian population, and our Prince and Ubelaker-based formula was also more accurate than the Lamendin et al. In both populations, the Lamendin method showed a higher mean error in estimations of the age of youngest and oldest individuals. These findings confirm the need to create specific formulas for each human group in order to obtain more accurate age estimates.